Home >> Antibodies >> ABCB11 / BSEP

ABCB11 / BSEP

Mouse Monoclonal Antibody [Clone BSEP/7534]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

SDS-PAGE Analysis Purified ABCB11 / BSEP Mouse Monoclonal Antibody (BSEP/7534). Confirmation of Purity and Integrity of Antibody.

Western blot analysis of THP-1 cell lysate using ABCB11 / BSEP Mouse Monoclonal Antibody (BSEP/7534).

Formalin-fixed, paraffin-embedded human liver stained with ABCB11 / BSEP Mouse Monoclonal Antibody (BSEP/7534). HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

SDS-PAGE Analysis Purified ABCB11 / BSEP Mouse Monoclonal Antibody (BSEP/7534). Confirmation of Purity and Integrity of Antibody.

Western blot analysis of THP-1 cell lysate using ABCB11 / BSEP Mouse Monoclonal Antibody (BSEP/7534).

Formalin-fixed, paraffin-embedded human liver stained with ABCB11 / BSEP Mouse Monoclonal Antibody (BSEP/7534). HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

Product Details

Synonyms

ABC member 16, MDR/TAP subfamily; ABC16; ABCB1; Abcb11; ATP binding cassette sub family B (MDR/TAP) member 11; ATP binding cassette sub family B member 11; Bile salt export pump (BSEP); BRIC2; PFIC2; PGY4; progressive familial intrahepatic cholestasis 2; Sister of P glycoprotein; Spgp

Positive Control

Human liver or adrenal gland.

Known Applications & Suggested Dilutions

Western Blot, IHC-P More Details

Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment (around aa600-800) of human ABCB11 protein (exact sequence is proprietary)

Cellular Localization

Membrane.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG

Mol. Weight of Antigen

160-190kDa

Specificity & Comments

ATP-binding cassette (ABC) transporters are an evolutionarily conserved family of proteins that catalyze the transport of molecules across extra- and intracellular membranes through the energy of ATP hydrolysis. ABC genes comprise seven subfamilies, designated ABC1, Mdr/TAP, MRP, ALD, OABP, GCN20 and White. The secretion of bile salt molecules from blood into bile is a major driving force for bile formation. Bile salt export pump (BSEP) is a member of the Mdr/TAP subfamily of ABC transporters that mediates the transport of bile acids across the hepatocyte canalicular membrane and regulates bile acid-dependent bile secretion. BSEP contains putative phosphorylation sites for protein kinase A, protein kinase C (PKC) and Ca2+-calmodulin dependent kinase II, whose regulation may be dependent on bile salt concentration.