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Bax (Apoptosis Marker)

Mouse Monoclonal Antibody [Clone BAX/962]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

Formalin-fixed, paraffin-embedded Hodgkin's Lymphoma stained with BAX Mouse Monoclonal Antibody (BAX/962).

Formalin-fixed, paraffin-embedded human Melanoma stained with BAX Mouse Monoclonal Antibody (BAX/962).

Flow Cytometric Analysis of Jurkat cells using Bax Mouse Monoclonal Antibody (Bax/962) followed by Goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).

Formalin-fixed, paraffin-embedded Hodgkin's Lymphoma stained with BAX Mouse Monoclonal Antibody (BAX/962).

Formalin-fixed, paraffin-embedded human Melanoma stained with BAX Mouse Monoclonal Antibody (BAX/962).

Flow Cytometric Analysis of Jurkat cells using Bax Mouse Monoclonal Antibody (Bax/962) followed by Goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).

Product Details

Synonyms

Apoptosis regulator BAX cytoplasmic isoform beta, Apoptosis regulator BAX membrane isoform alpha, Bax isoform psi, Bax protein cytoplasmic isoform delta, Bax protein cytoplasmic isoform gamma, Bax zeta, Bcl-2-like protein 4, BCL2 associated X protein, BCL2 associated X protein omega, BCL2 associated X protein transcript variant delta2, BCL2L4

Positive Control

Jurkat, K562, HL-60 or HeLa cells. Reed-Sternberg cells in Hodgkin's lymphoma.

Known Applications & Suggested Dilutions

Flow Cyt, IHC-P More Details

Flow Cytometry (1-2ug/million cells)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA buffer, pH 8.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human BAX protein.

Cellular Localization

Cytoplasmic

Species Reactivity

Human. Monkey. Does not react with Mouse or Rat.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

21kDa

Specificity & Comments

Recognizes a protein of 21kDa, identified as the Bax protein. This MAb is highly specific to Bax and shows no cross-reaction with Bcl-2 or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2.