Home >> Antibodies >> CD15 / FUT4 (Reed-Sternberg Cell Marker)

CD15 / FUT4 (Reed-Sternberg Cell Marker)

Mouse Monoclonal Antibody [Clone SPM119]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

Formalin-fixed, paraffin-embedded human Hodgkin's Lymphoma stained with CD15 Monoclonal Antibody (SPM119).

FACS analysis of CD15 on human Monocytes using CD15 Monoclonal Antibody (SPM119).

Immunofluorescence staining of U937 cells using CD15 Monoclonal Antibody (SPM119) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.

Flow Cytometric Analysis of U937 cells using CD15 MAb (SPM119) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Formalin-fixed, paraffin-embedded human Hodgkin's Lymphoma stained with CD15 Monoclonal Antibody (SPM119).

FACS analysis of CD15 on human Monocytes using CD15 Monoclonal Antibody (SPM119).

Immunofluorescence staining of U937 cells using CD15 Monoclonal Antibody (SPM119) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.

Flow Cytometric Analysis of U937 cells using CD15 MAb (SPM119) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Product Details

Synonyms

3 Fucosyl N Acetyl Lactosamine; Alpha (1,3) Fucosyltransferase; Alpha 13 fucosyltransferase FucT; ELAM Ligand Fucosyltransferase; ELFT; FCT3A; Fuc-TIV; Fucosyltransferase 4 Alpha 1 3 Fucosyltransferase Myeloid Specific; Fucosyltransferase 4; Galactoside 3 L Fucosyltransferase; Lewis X; LeX; SSEA1; Stage Specific Embryonic Antigen 1

Positive Control

U937 cells, Reed-Sternberg's cells in Hodgkin's lymphoma.

Known Applications & Suggested Dilutions

Flow Cyt, IF, IHC-P More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA, pH 7.5-8.5, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

U937 histiocytic cell line

Cellular Localization

Cell surface and granular paranuclear

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgM, kappa

Mol. Weight of Antigen

~220kDa

Specificity & Comments

CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on 95% of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. In addition, CD15 is expressed in Reed-Sternberg cells and some epithelial cells. CD15 antibody is very useful in the identification of Hodgkin s disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance.