Home >> Antibodies >> CD171 / NCAM-L1 (Axonal Marker)

CD171 / NCAM-L1 (Axonal Marker)

Mouse Monoclonal Antibody [Clone UJ127]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

SDS-PAGE Analysis Purified CD171 Mouse Monoclonal Antibody (UJ127). Confirmation of Integrity and Purity of Antibody.

Formalin-fixed, paraffin-embedded human cerebellum stained with CD171 Mouse Monoclonal Antibody (UJ127). Inset: PBS instead of primary antibody; secondary only negative control.

Formalin-fixed, paraffin-embedded human ovarian cancer stained with CD171 Mouse Monoclonal Antibody (UJ127). Inset: PBS instead of primary antibody; secondary only negative control.

Formalin-fixed, paraffin-embedded human prostate carcinoma stained with CD171 Mouse Monoclonal Antibody (UJ127). HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

SDS-PAGE Analysis Purified CD171 Mouse Monoclonal Antibody (UJ127). Confirmation of Integrity and Purity of Antibody.

Formalin-fixed, paraffin-embedded human cerebellum stained with CD171 Mouse Monoclonal Antibody (UJ127). Inset: PBS instead of primary antibody; secondary only negative control.

Formalin-fixed, paraffin-embedded human ovarian cancer stained with CD171 Mouse Monoclonal Antibody (UJ127). Inset: PBS instead of primary antibody; secondary only negative control.

Formalin-fixed, paraffin-embedded human prostate carcinoma stained with CD171 Mouse Monoclonal Antibody (UJ127). HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

Product Details

Synonyms

CAML1, HSAS1, Hyd, L1 Cell Adhesion Molecule, L1-NCAM, MASA, MIC5, NCAM-L1, Nerve-growth factor-inducible large external glycoprotein, Neural cell adhesion molecule L1, NILE, S10, SPG1

Positive Control

Human neuroblastomas or schwannomas.

Known Applications & Suggested Dilutions

Flow Cyt, IF More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml),Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Homogenous suspension of 16-week human fetal brain

Cellular Localization

Cell surface.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

220-240kDa

Specificity & Comments

Recognizes a cell surface protein of 220-240kDa, identified as L1 cell adhesion molecule. The L1CAM gene, which is located in Xq28, is involved in three distinct conditions: 1) HSAS (hydrocephalus-stenosis of the aqueduct of Sylvius); 2) MASA (mental retardation, aphasia, shuffling gait, and adducted thumbs); and 3) SPG1 (spastic paraplegia). The L1, neural cell adhesion molecule (L1CAM) also plays an important role in axon growth, fasciculation, and neural migration as well as in mediating neuronal differentiation. Expression of L1 protein is restricted to tissues arising from neuroectoderm. This MAb is useful in the identification of primitive neuroectodermal tumors. It binds to tumors of neuroectodermal and glial origin e.g. neuroblastoma and Schwannomas. It does not bind to pediatric or adult brain.