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Home >> Antibodies >> DNMT3A / DNA Methyltransferase 3 Alpha

DNMT3A / DNA Methyltransferase 3 Alpha

Mouse Monoclonal Antibody [Clone PCRP-DNMT3A-1E2]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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Formalin-fixed, paraffin-embedded human Placenta stained with DNMT3A Mouse Monoclonal Antibody (PCRP-DNMT3A-1E2).

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SDS-PAGE Analysis Purified DNMT3A Mouse Moncolonal Antibody (PCRP-DNMT3A-1E2). Confirmation of Purity and Integrity of Antibody.

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Formalin-fixed, paraffin-embedded human Placenta stained with DNMT3A Mouse Monoclonal Antibody (PCRP-DNMT3A-1E2).

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Analysis of Protein Array containing more than 19,000 full-length human proteins using DNMT3A Mouse Monoclonal Antibody (PCRP-DNMT3A-1E2)

Product Details

Synonyms

DNA methyltransferase 3 alpha; DNA methyltransferase HsaIIIA; DNA MTase HsaIIIA; DNMT; DNMT3A2; M.HsaIIIA; MCMT; TBRS

Positive Control

HeLa cells. Placenta.

Known Applications & Suggested Dilutions

Western Blot, IHC-P         More Details

Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human full-length DNMT3A protein

Cellular Localization

Nuclear

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

102kDa

Specificity & Comments

The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. DNMT3A is required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZBTB18. Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites. Can actively repress transcription through the recruitment of HDAC activity. Sadenosyl-L-methionin

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200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Bioinformatics

  •  Entrez Gene ID: 1788
  •  Gene Symbol: DNMT3A
  •  SwissProt: Q16655
  •  Chromosome Location: 2p23.3
  •  Unigene: 154069
NBT01898