Home >> Antibodies >> MART-1 / Melan-A / MLANA (Melanoma Marker)

MART-1 / Melan-A / MLANA (Melanoma Marker)

Recombinant Mouse Monoclonal Antibody [Clone rMLANA/788]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

Formalin-fixed, paraffin-embedded human Melanoma stained with MART-1 Mouse Recombinant Monoclonal Antibody (rMLANA/788).

SDS-PAGE Analysis of Purified MART-1 Mouse Recombinant Monoclonal Antibody (rMLANA/788). Confirmation of Purity and Integrity of Antibody.

Western Blot Analysis of COLO38 cell lysate using MART-1 Mouse Recombinant Monoclonal Antibody (rMLANA/788).

Formalin-fixed, paraffin-embedded human Melanoma stained with MART-1 Mouse Recombinant Monoclonal Antibody (rMLANA/788).

SDS-PAGE Analysis of Purified MART-1 Mouse Recombinant Monoclonal Antibody (rMLANA/788). Confirmation of Purity and Integrity of Antibody.

Western Blot Analysis of COLO38 cell lysate using MART-1 Mouse Recombinant Monoclonal Antibody (rMLANA/788).

Product Details

Synonyms

Antigen LB39-AA, Antigen SK29-AA, Melanoma antigen recognized by T-cells 1, MLAN-A, MLANA

Positive Control

COLO38, SK-MEL-13 and SK-MEL-19 melanoma cell lines. Human melanoma tissues.

Known Applications & Suggested Dilutions

Western Blot, IHC-P More Details

Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length hMART-1 protein

Cellular Localization

Cytoplasmic

Species Reactivity

Human. Mouse. Rat. Dog.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

20-22kDa (doublet)

Specificity & Comments

This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.