Home >> Antibodies >> MyoD1 (Rhabdomyosarcoma Marker)

MyoD1 (Rhabdomyosarcoma Marker)

Mouse Monoclonal Antibody [Clone 5.8A]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

Formalin-fixed, paraffin-embedded human rhabdomyosarcoma stained with MyoD1 Mouse Monoclonal Antibody (5.8).

SDS-PAGE Analysis Purified MyoD1 Mouse Monoclonal Antibody (5.8A). Confirmation of Integrity and Purity of Antibody.

Formalin-fixed, paraffin-embedded human rhabdomyosarcoma stained with MyoD1 Mouse Monoclonal Antibody (5.8).

SDS-PAGE Analysis Purified MyoD1 Mouse Monoclonal Antibody (5.8A). Confirmation of Integrity and Purity of Antibody.

Formalin-fixed, paraffin-embedded human rhabdomyosarcoma stained with MyoD1 Mouse Monoclonal Antibody (5.8).

Product Details

Synonyms

bHLHc1, Class C basic helix-loop-helix protein 1, Myoblast determination protein 1, Myogenic differentiation 1, Myogenic factor 3 (Myf-3), Myogenin D1, PUM

Positive Control

Rhabdomyosarcoma

Known Applications & Suggested Dilutions

Flow Cyt, IF, IHC-P More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant mouse MyoD1 protein

Cellular Localization

Nucleus. Only nuclear staining should be considered as evidence of skeletal muscle differentiation.

Species Reactivity

Human. Mouse. Rat. Chicken.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

45kDa

Specificity & Comments

Recognizes a phosphor-protein of 45kDa, identified as MyoD1. The epitope of this MAb maps between amino acid 180-189 in the C-terminal of mouse MyoD1 protein. It does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilm s tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewing s sarcomas and alveolar soft part sarcomas.