Home >> Antibodies >> N-Cadherin / Cadherin-2 / CD325 (NCAD)

N-Cadherin / Cadherin-2 / CD325 (NCAD)

Rat Monoclonal Antibody [Clone MNCD2]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

Formalin-fixed, paraffin-embedded human heart stained with N-Cadherin Rat Monoclonal Antibody (MNCD2).

Formalin-fixed, paraffin-embedded human heart stained with N-Cadherin Rat Monoclonal Antibody (MNCD2).

Product Details

Synonyms

Cadherin-2 N cadherin neuronal; Cadherin-2 type 1; Cadherin-2; Calcium dependent adhesion protein neuronal; CD325; CDH2; CDHN; CDw325; N-Cadherin; NCAD

Positive Control

Human heart, pancreas or cerebral cortex (IHC).

Known Applications & Suggested Dilutions

Flow Cyt, Western Blot, IHC-P More Details

Flow Cytometry (1-2ug/million cells)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment (aa308-597) around mouse CDH2. The epitope is the extracellular domain of N-cadherin.

Cellular Localization

Cell surface

Species Reactivity

Human, mouse and chick.

Host / Ig Isotype

Rat / IgG2a

Mol. Weight of Antigen

99.76kDa

Specificity & Comments

Recognizes a protein of ~140kDa, identified as N-Cadherin (NCAD), also known as CD325. NCAD is a member of the Cadherin superfamily, and consists of five extracellular repeats, a transmembrane domain and a cytoplasmic domain. CD325 deficient mice die at day 10 of gestation and embryos display major heart defects and malformed neural tubes and somites. Consistent with this, CD325 has been implicated in several aspects of cardiac development including the precardiac mesoderm, establishment of left-right symmetry and cardiac looping morphogenesis. Furthermore, CD325 is normally involved in inducing cell cycle arrest and its expression is frequently deregulated in cancer cells.Studies have linked N-cadherin to cancer metastasis by showing the aggressive tumor cells had preferentially turned on N-cadherin as opposed to E- or P-cadherin.