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Home >> Antibodies >> PDGF beta (PDGFB)

PDGF beta (PDGFB)

Mouse Monoclonal Antibody [Clone PDGFB/3071]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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Formalin-fixed, paraffin-embedded human colon stained with PDGF beta Mouse Monoclonal Antibody (PDGFB/3071). Inset: PBS instead of primary antibody, secondary antibody negative control.

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SDS-PAGE Analysis Purified PDGF beta Mouse Monoclonal Antibody (PDGFB/3071). Confirmation of Purity and Integrity of Antibody.

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Analysis of Protein Array containing more than 19,000 full-length human proteins using PDGF beta Mouse Monoclonal Antibody (PDGFB/3071).

Product Details

Synonyms

Becaplermin; c sis; Oncogene SIS; PDGF Beta chain; PDGF2; Platelet derived growth factor 2; Platelet derived growth factor B chain; Platelet derived growth factor beta; Platelet derived growth factor beta polypeptide (oncogene SIS); Platelet derived growth factor beta polypeptide (simian sarcoma viral (v sis) oncogene homolog)

Positive Control

Human colon, kidney or lymph node.

Known Applications & Suggested Dilutions

IHC-P         More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment of human PDGFB protein (around aa 27-158) (exact sequence is proprietary)

Cellular Localization

Cytoplasm.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2c, kappa

Mol. Weight of Antigen

14kDa (monomeric B chain); 31-35kDa (dimer)

Specificity & Comments

PDGF is a mitogen for mesenchyme- and glia-derived cells. It consists of two disulfide-bonded polypeptide chains, A and B, and occurs as three isoforms; PDGF AA, AB and BB. The three isoforms bind, with different affinities, to two receptor types, and , which are structurally related and endowed with protein-tyrosine kinase domains. Ligand binding induces activation of the receptor kinases by formation of receptor dimers; the A subunit of PDGF binds only to receptors with high affinity, whereas the B subunit can bind to both and receptors. Evidence suggests that PDGF may function as a neurotrophic factor. Receptors for PDGF-A are expressed in oligodendrocyte progenitor cells whereas receptors for PDGF-B are expressed on neurons. These facts suggest that the different isoforms of PDGF may regulate growth and differentiation of different cell types in the developing central nervous system through paracrine and autocrine routes.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Ross, R., et al. 1986. The biology of platelet-derived growth factor. Cell46: 155-169.

Bioinformatics

  •  Entrez Gene ID: 5155
  •  Gene Symbol: PDGFB
  •  SwissProt: P01127
  •  Chromosome Location: 22q13.1
  •  Unigene: 1976