Home >> Antibodies >> QKI / Protein Quaking

QKI / Protein Quaking

Mouse Monoclonal Antibody [Clone PCRP-QKI-2F10]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

SDS-PAGE Analysis Purified QKI Mouse Monoclonal Antibody (PCRP-QKI-2F10) Confirmation of Purity and Integrity of Antibody.

Flow cytometric analysis of PFA-fixed HeLa cells. QKI Mouse Monoclonal Antibody (PCRP-QKI-2F10) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Flow cytometric analysis of PFA-fixed K562 cells. QKI Mouse Monoclonal Antibody (PCRP-QKI-2F10) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Flow cytometric analysis of PFA-fixed U87 cells. QKI Mouse Monoclonal Antibody (PCRP-QKI-2F10) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Flow cytometric analysis of PFA-fixed MCF7 cells. QKI Mouse Monoclonal Antibody (PCRP-QKI-2F10) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Immunofluorescence Analysis of PFA-fixed U87 cells. Staining localized to the nucleoplasm using QKI Mouse Monoclonal Antibody (PCRP-QKI-2F10) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).

Immunofluorescence Analysis of K562 cells stained using QKI Mouse Monoclonal Antibody (PCRP-QKI-2F10) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).

Immunofluorescence Analysis of PFA-fixed MCF7 cells stained using QKI Mouse Monoclonal Antibody (PCRP-QKI-2F10) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).

Analysis of Protein Array containing more than 19,000 full-length human proteins using QKI-Monospecific Mouse Monoclonal Antibody (PCRP-QKI-2F10).

Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

SDS-PAGE Analysis Purified QKI Mouse Monoclonal Antibody (PCRP-QKI-2F10) Confirmation of Purity and Integrity of Antibody.

Flow cytometric analysis of PFA-fixed HeLa cells. QKI Mouse Monoclonal Antibody (PCRP-QKI-2F10) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Flow cytometric analysis of PFA-fixed K562 cells. QKI Mouse Monoclonal Antibody (PCRP-QKI-2F10) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Flow cytometric analysis of PFA-fixed U87 cells. QKI Mouse Monoclonal Antibody (PCRP-QKI-2F10) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Flow cytometric analysis of PFA-fixed MCF7 cells. QKI Mouse Monoclonal Antibody (PCRP-QKI-2F10) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Immunofluorescence Analysis of PFA-fixed U87 cells. Staining localized to the nucleoplasm using QKI Mouse Monoclonal Antibody (PCRP-QKI-2F10) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).

Immunofluorescence Analysis of K562 cells stained using QKI Mouse Monoclonal Antibody (PCRP-QKI-2F10) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).

Immunofluorescence Analysis of PFA-fixed MCF7 cells stained using QKI Mouse Monoclonal Antibody (PCRP-QKI-2F10) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).

Analysis of Protein Array containing more than 19,000 full-length human proteins using QKI-Monospecific Mouse Monoclonal Antibody (PCRP-QKI-2F10).

Product Details

Synonyms

Homolog of mouse quaking QKI KH domain RNA binding protein; HQK1; Protein quaking; QK1; QK3; QKI; QKI1; Quaking homolog KH domain RNA binding mouse; Quaking isoform 1; Quaking protein; RNA binding protein HQK

Positive Control

U87, HeLa, K562 or MCF7 cells.

Known Applications & Suggested Dilutions

ELISA, Flow Cyt, IP, IF, Western Blot More Details

ELISA (concentration assay dependent)
Flow Cytometry (1-2ug/million cells)
Immunoprecipitation (1-2ug per 100-500ug of total protein (1ml of cell lysate))
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human QKI protein

Cellular Localization

Nucleus. Cytoplasm.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2c

Mol. Weight of Antigen

37.6kDa

Specificity & Comments

QKI, also known as HKQ, QK, QK3 or quaking, is a 341 amino acid protein that localizes to both the cytoplasm and the nucleus and contains one KH domain. Expressed in the frontal cortex of the brain, QKI functions as an RNA-binding protein that plays an important role in myelinization and specifically binds to the RNA core sequence 5'-NACUAAY-N(1,20)-UAAY-3'. Additionally, QKI regulates pre-mRNA splicing, and mRNA export and is involved in protecting and promoting the stability of select mRNAs. QKI may be methylated by PRMT1 and may also be phosphorylated at its C-terminus, an event that decreases QKI mRNA-binding affinity. Defects or deletions in the gene encoding QKI are associated with astrocytic tumors and may be involved in the pathogenesis of schizophrenia. Multiple isoforms of QKI exist due to alternative splicing events.