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Home >> Antibodies >> Ras-related C3 botulinum toxin substrate 1

Ras-related C3 botulinum toxin substrate 1

Mouse Monoclonal Antibody [Clone CPTC-RAC1-1]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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SDS-PAGE Analysis Purified RAC1Mouse Monoclonal Antibody (CPTC-RAC1-1). Confirmation of Integrity and Purity of Antibody.

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Formalin-fixed, paraffin-embedded human lung stained with RAC1Mouse Monoclonal Antibody (CPTC-RAC1-1).

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Formalin-fixed, paraffin-embedded human lung stained with RAC1Mouse Monoclonal Antibody (CPTC-RAC1-1).

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Analysis of Protein Array containing more than 19,000 full-length human proteins using Ras-related C3 botulinum toxin substrate 1Monoclonal Antibody (CPTC-RAC1-1).

Product Details

Synonyms

Cell migration-inducing gene 5 protein, MIG5, Rac-1, Ras-like protein TC25, Ras-related C3 botulinum toxin substrate 1

Positive Control

HeLa or Jurkat cells. Human breast, prostate or tonsil tissues.

Known Applications & Suggested Dilutions

ELISA, IF, Western Blot, IHC-P         More Details

ELISA (For coating, order antibody without BSA)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human full-length RAC1protein

Cellular Localization

Cell surface. Melanosome.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

21kDa

Specificity & Comments

Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages (By similarity). Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. Rac1 has been demonstrated to play a critical role in tumor progression of human colorectal adenocarcinoma cells. In one study, overexpression of Rac1 accelerated the tumorigenic process, whereas Rac1 inhibition completed suppressed tumor formation.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Gao, Y., et al. Proc. Natl. Acad. Sci. U.S.A. (2004)
  2. Espina, C., et al. Am. J. Pathol. (2008)

Bioinformatics

  •  Entrez Gene ID: 5879
  •  Gene Symbol: RAC1
  •  SwissProt: P63000
  •  Chromosome Location: 7p22.1
  •  Unigene: 413812
NBT02264