Home >> Antibodies >> RCAS1 / Estrogen Receptor Binding Site Associated, Antigen 9

RCAS1 / Estrogen Receptor Binding Site Associated, Antigen 9

Mouse Monoclonal Antibody [Clone CPTC-EBAG9-2]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

Formalin-fixed, paraffin-embedded human Tonsil stained with RCAS1 Mouse Monoclonal Antibody (CPTC-EBAG9-2).

SDS-PAGE Analysis Purified RCAS1 Mouse Monoclonal Antibody (CPTC-EBAG9-2). Confirmation of Purity and Integrity of Antibody.

Western Blot Analysis of HeLa cell lysate using RCAS1 Mouse Monoclonal Antibody (CPTC-EBAG9-2).

Formalin-fixed, paraffin-embedded human Tonsil stained with RCAS1 Mouse Monoclonal Antibody (CPTC-EBAG9-2).

SDS-PAGE Analysis Purified RCAS1 Mouse Monoclonal Antibody (CPTC-EBAG9-2). Confirmation of Purity and Integrity of Antibody.

Western Blot Analysis of HeLa cell lysate using RCAS1 Mouse Monoclonal Antibody (CPTC-EBAG9-2).

Product Details

Synonyms

BAG9; cancer associated surface antigen; cancer associated surface antigen RCAS1; EB9; estrogen receptor binding fragment associated gene 9; PDAF; receptor binding cancer antigen expressed on SiSo cells

Positive Control

MCF-7, Jurkat or HEK-293 cell lysates. Human skeletal muscle tissue.

Known Applications & Suggested Dilutions

IF, Western Blot, IHC-P More Details

Immunofluorescence (1-2ug/ml)
Western Blot (0.5-1ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human EBAG9 protein

Cellular Localization

Golgi apparatus.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2a, kappa

Mol. Weight of Antigen

32kDa

Specificity & Comments

EBAG9, also known as RCAS1, is an estrogen-transcribed protein. Soluble and membranous RCAS1 proteins may play a role in the immune escape of tumor cells by promoting T lymphocyte inhibition of growth and apoptosis. RCAS1 is expressed in a wide variety of cancers, including uterine, ovarian, and lung cancer cells, and acts as a ligand for a putative receptor present on peripheral lymphocytes. RCAS1 is highly expressed not only in cancer cells but also in non-tumor bile duct cells subject to immune attack. RCAS1 inhibits the in vitro growth of receptor-expressing cells and induces apoptosis, contributing to the ability of tumor cells to evade host immune surveillance. High expression of RCAS1 significantly correlates with tumor progression and with poor outcome for many cancer patients.