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Home >> Antibodies >> Sirtuin 3 (SIRT3)

Sirtuin 3 (SIRT3)

Mouse Monoclonal Antibody [Clone PCRP-SIRT3-1C10]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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Flow cytometric analysis of PFA-fixed HeLa cells. SIRT3 Mouse Monoclonal Antibody (PCRP-SIRT3-1C10) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

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SDS-PAGE Analysis Purified SIRT3 Mouse Monoclonal Antibody (PCRP-SIRT3-1C10). Confirmation of Purity and Integrity of Antibody.

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Immunofluorescence Analysis of PFA-fixed HeLa cells stained using SIRT3 Mouse Monoclonal Antibody (PCRP-SIRT3-1C10) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

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Analysis of Protein Array containing more than 19,000 full-length human proteins using SIRT3-Monospecific Mouse Monoclonal Antibody (PCRP-SIRT3-1C10).

Product Details

Synonyms

hSIRT3; Mitochondrial nicotinamide adenine dinucleotide dependent deacetylase; NAD dependent deacetylase sirtuin 3 mitochondrial; Regulatory protein SIR2 homolog 3; SIR2 like protein 3; SIR2-like protein 3; SIR2L3; Sirtuin silent mating type information regulation 2 homolog 3 (S. cerevisiae); Sirtuin type 3; Sirtuin3

Positive Control

HeLa cells.

Known Applications & Suggested Dilutions

ELISA, IP, Flow Cyt, IF, Western Blot         More Details

ELISA (For coating, order antibody without BSA)
Immunoprecipitation (1-2ug per 100-500ug of total protein (1ml of cell lysate))
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human SIRT3 protein

Cellular Localization

Mitochondrion matrix.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2a

Mol. Weight of Antigen

43.57kDa

Specificity & Comments

Zinc-fingers and homeobox (ZHX) proteins are transcription factors that interact with the activation domain of the A subunit of nuclear factor-Y (NF-YA). ZHX1-3 are ubiquitously expressed proteins expressed in various tissues. They act as transcriptional repressors and localize to the nucleus. The ZHX proteins contain two Cys2-His2-type zinc-finger motifs and five homeodomains (HDs). These domains allow the ZHX proteins to form homodimers, but they can also form heterodimers with each other. However, this dimerization is not required for repressor activity. Hypermethylation-mediated silencing of ZHX2 is an epigenetic event involved in hepatocellular carcinoma (HCC).

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200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Blackshaw S, et al. Nature methods 15.5 (2018 May): 330-338.

Bioinformatics

  •  Entrez Gene ID: 23410
  •  Gene Symbol: SIRT3
  •  SwissProt: Q9NTG7
  •  Chromosome Location: 20q12
  •  Unigene: 716456