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Home >> Antibodies >> Steroidogenic Factor 1 (SF-1) (Transcription Factor)

Steroidogenic Factor 1 (SF-1) (Transcription Factor)

Mouse Monoclonal Antibody [Clone NR5A1/3420]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
img

Formalin-fixed, paraffin-embedded human testis stained with SF-1 Mouse Monoclonal Antibody (NR5A1/3420) at 2ug/ml. Inset: PBS instead of primary antibody; secondary only negative control.

img

Analysis of Protein Array containing >19,000 full-length human proteins using Steroidogenic Factor 1 (SF-1) Mouse Monoclonal Antibody (NR5A1/3420).

Product Details

Synonyms

Adrenal 4 binding protein (AD4BP); ELP; Fushi tarazu factor homolog 1 (FTZ1); Nuclear receptor AdBP4; Nuclear receptor subfamily 5 group A member 1 (NR5A1); POF7; SPGF8; SRXY3; Steroid hormone receptor Ad4BP; Steroidogenic factor 1 (SF1); Steroidogenic factor 1 nuclear receptor; STF1

Positive Control

Human testis or adrenal cortical carcinoma.

Known Applications & Suggested Dilutions

IHC-P         More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment (around aa220-461) of human SF-1 protein

Cellular Localization

Nucleus.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2b, kappa

Mol. Weight of Antigen

53kDa

Specificity & Comments

The protein encoded by this gene is a transcriptional activator involved in sex determination. The encoded protein binds DNA as a monomer. Defects in this gene are a cause of XY sex reversal with or without adrenal failure as well as adrenocortical insufficiency without ovarian defect. Steroidogenic Factor 1 (SF-1) is considered an orphan nuclear receptor that belongs to subfamily 5. It was found to be a regulator of steroidogenic enzyme gene expression. Oxysterols are suggested as its ligands. It is expressed in all steroidogenic tissues, including the adrenal cortex, testicular Sertoli cells, and Leydig cells, ovarian theca, hypothalamus, and anterior pituitary. SF-1 plays an important role in adrenal and gonadal development. SF-1 is highly valuable marker to determine the adrenocortical origin of an adrenal mass.

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200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Zhao C, Vinh TN, et al. Am J Surg Pathol. 2009; 33:354-66.
  2. Sangoi AR, et al. AmJ Surg Pathol. 2011; 35:678-86.

Bioinformatics

  •  Entrez Gene ID: 2516
  •  Gene Symbol: NR5A1
  •  SwissProt: Q13285
  •  Chromosome Location: 9q33.3
  •  Unigene: 495108