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Synaptophysin (Neuroendocrine Marker)

Recombinant Mouse Monoclonal Antibody [Clone rSYP/6856]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

Formalin-fixed, paraffin-embedded human brain stained with rSYP/6856 at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min. Inset: PBS instead of primary. Secondary antibody negative control.

SDS-PAGE Analysis Purified Synaptophysin Recombinant Mouse Monoclonal Antibody (rSYP/6856). Confirmation of Purity and Integrity of Antibody.

Formalin-fixed, paraffin-embedded human brain stained with rSYP/6856 at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min. Inset: PBS instead of primary. Secondary antibody negative control.

SDS-PAGE Analysis Purified Synaptophysin Recombinant Mouse Monoclonal Antibody (rSYP/6856). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

Major synaptic vesicle protein p38; MRX96; MRXSYP; Syn p38; Syp; SYPH

Positive Control

HepG2, HeLa or Y79 cells. Human pancreas, cerebellum or pheochromocytoma.

Known Applications & Suggested Dilutions

IHC-P More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment (around aa 224-313) of human Synaptophysin (SYP) protein (exact sequence is proprietary)

Cellular Localization

Cytoplasm.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

38kDa

Specificity & Comments

This recombinant mouse monoclonal antibody recognizes a protein of 38kDa that is identified as synaptophysin. It is an N-glycosylated integral membrane protein found in neurons and endocrine cells. Synaptophysin contains four transmembrane domains and may function as a gap junction-like channel. This antibody identifies normal neuroendocrine cells and neuroendocrine neoplasms. Diffuse, finely granular, cytoplasmic staining is observed, which probably correlates with the distribution of the antigen within neurosecretory vesicles. Synaptophysin is an independent, broad-range marker of neural and neuroendocrine differentiation.