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TIM3 / HAVCR2/ CD366 (Effector T-Cell Marker)

Mouse Monoclonal Antibody [Clone TIM3/4024]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

Western blot analysis of human spleen tissue lysate using TIM3 Mouse Monoclonal Antibody (TIM3/4024).

Formalin-fixed, paraffin-embedded human lymph node stained with TIM3 Mouse Monoclonal Antibody (TIM3/4024).

Formalin-fixed, paraffin-embedded human renal carcinoma stained with TIM3 Mouse Monoclonal Antibody (TIM3/4024).

Formalin-fixed, paraffin-embedded human lymph node stained with TIM3 Mouse Monoclonal Antibody (TIM3/4024).

Western blot analysis of human spleen tissue lysate using TIM3 Mouse Monoclonal Antibody (TIM3/4024).

Formalin-fixed, paraffin-embedded human lymph node stained with TIM3 Mouse Monoclonal Antibody (TIM3/4024).

Formalin-fixed, paraffin-embedded human renal carcinoma stained with TIM3 Mouse Monoclonal Antibody (TIM3/4024).

Formalin-fixed, paraffin-embedded human lymph node stained with TIM3 Mouse Monoclonal Antibody (TIM3/4024).

Product Details

Synonyms

CD366; HAVR2; Hepatitis A virus cellular receptor 2 (HAVCR2); Kidney injury molecule 3 (KIM3); T-cell immunoglobulin and mucin domain-containing protein 3; T-cell immunoglobulin mucin receptor 3; T-cell membrane protein 3; TIM3; TIMD3

Positive Control

HepG2or Daudi cells. Human tonsil, lymph node or spleen.

Known Applications & Suggested Dilutions

Western Blot, IHC-P More Details

Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment of human TIM3 protein (around aa 22-202) (exact sequence is proprietary)

Cellular Localization

Cell Surface

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2b, kappa

Mol. Weight of Antigen

60kDa

Specificity & Comments

TIMs are type I transmembrane glycoproteins with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk. TIM-3 is expressed on the surface of effector T cel tonsil, lymph node or spleen. ls (CD4+Th1 and CD8+Tc1) but not on helper T cells (CD4+Th2 and CD8+Tc2). In chronic inflammation, autoimmune disorders, and some cancers, TIM-3 is upregulated on several other hematopoietic cell types. The Ig domain of TIM-3 interacts with a ligand on resting but not activated Th1 and Th2 cells. The glycosylated Ig domain of TIM-3 binds cell-associated galectin-9. This induces TIM-3 Tyr phosphorylation and pro-apoptotic signaling. TIM-3 functions as a negative regulator of Th1 cell activity. Its blockade results in increased IFN-gamma production, Th1 cell proliferation and cytotoxicity, regulatory T cell development, and increases in macrophage and neutrophil infiltration into sites of inflammation.