Home >> Antibodies >> Transglutaminase II (TGM2) (FN Binding Domain)

Transglutaminase II (TGM2) (FN Binding Domain)

Mouse Monoclonal Antibody [Clone TGM2/3612]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

Formalin-fixed, paraffin-embedded human placenta stained with Transglutaminase II Mouse Monoclonal Antibody (TGM2/3612). Inset: PBS instead of primary antibody; secondary only negative control.

SDS-PAGE Analysis Purified Transglutaminase II Mouse Monoclonal Antibody (TGM2/3612). Confirmation of Purity and Integrity of Antibody.

Formalin-fixed, paraffin-embedded human placenta stained with Transglutaminase II Mouse Monoclonal Antibody (TGM2/3612). Inset: PBS instead of primary antibody; secondary only negative control.

SDS-PAGE Analysis Purified Transglutaminase II Mouse Monoclonal Antibody (TGM2/3612). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

Protein-glutamine gamma-glutamyltransferase 2; TG2; TGase C; TGase H; TGase-2; TgaseII; TGC; TGM2; Tissue type transglutaminase; Transglutaminase 2 C polypeptide; Transglutaminase H; tTG; tTGas

Positive Control

HUVEC cells.Endothelial cells in placenta, liver, brain, or breast carcinoma. Smooth muscle cells of any origin.

Known Applications & Suggested Dilutions

Flow Cyt, IF, IHC-P More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human TGM2 protein

Cellular Localization

Cytoplasm. Cell surface.

Species Reactivity

Human, Mouse and Rat.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

77-85kDa

Specificity & Comments

Tissue transglutaminase (tTG) is a 75 kDa monomeric globular protein expressed in the majority of cells and tissues. tTG localizes mainly in the cytoplasm but some tGT is present on the cell surface and is association with the extracellular matrix. In addition to its guanosine triphosphatase (GTPase) activity, tTG catalyzes the posttranslational modification of proteins by transamidation of available glutamine residues (Monsonego, 1997). Extracellular tTG is able to bind to and cross-link several ECM proteins and may function to stabilize the ECM and basement membranes. The protein is translated as a fully active enzyme and there is no evidence for proteolytic activation.High constitutive expression and accumulation of active tTG is observed in (among others) endothelial cells, platelets, vascular smooth muscle cells and epithelial cells of the lens. tTG is expressed at very low levels in skeletal muscle cells and neurons and may be difficult to detect in these cell/tissue types.