2 Union Square, Union City, CA 94587
contact@NeoBiotechnologies.com
510-376-5603

Home >> Antibodies >> TRBP2 / TARBP2

TRBP2 / TARBP2

Mouse Monoclonal Antibody [Clone PCRP-TARBP2-1E5]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
img

Flow Cytometric Analysis of PFA-fixed HeLa cells. TRBP2 / TARBP2 Mouse Monoclonal Antibody (PCRP-TARBP2-1E5) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).

img

Immunofluorescence Analysis of PFA-fixed HeLa cells using TRBP2 / TARBP2 Mouse Monoclonal Antibody (PCRP-TARBP2-1E5) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).

img

Analysis of Protein Array containing more than 19,000 full-length human proteins using TRBP2 / TARBP2 Mouse Monoclonal Antibody (PCRP-TARBP2-1E5).

img

SDS-PAGE Analysis. Purified TRBP2 / TARBP2 Mouse Monoclonal Antibody (PCRP-TARBP2-1E5). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

LOQS; Prbp; RISC loading complex subunit TARBP2; TAR (HIV 1) RNA binding protein 2; TAR RNA Binding Protein 2; TARBP2 RISC loading complex RNA binding subunit; Trans-activation-responsive RNA-binding protein; TRBP; TRBP1

Positive Control

MCF-7 or HeLa cells.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human TARBP2 protein

Cellular Localization

Cytoplasm. Cytoplasm > perinuclear region. Nucleus.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2a

Mol. Weight of Antigen

45kDa

Specificity & Comments

TRBP2, also known as TARBP2 (trans-activation-responsive (HIV-1) RNA binding protein 2), TRBP1 or TRBP, is a nuclear protein that contains three DRBM (double-stranded RNA-binding) domains. TRBP binds between the bulge and the loop of the HIV-1 TAR RNA regulatory element and activates HIV-1 gene expression in synergy with the viral Tat protein. The third DRBM motif in the C-terminus of human TRBP2 can interact with and inhibit PKR activity, thereby increasing HIV-1 long terminal repeat (LTR) expression. In addition, TRBP2 functions as a component of a Dicer-containing complex and associates with the catalytic subunit of the RNA-induced silencing complex (RISC), namely eIF2C2. TRBP2 is essential for Dicer stability and the proper assembly of RISC. This suggests that TRBP2, in association with Dicer, plays an important role in the processing of miRNAs (microRNAs).

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Tian T, et al. Long Noncoding RNA MPRL Promotes Mitochondrial Fission and Cisplatin Chemosensitivity via Disruption of Pre-miRNA Processing. Clin Cancer Res 25:3673-3688 (2019).

Bioinformatics

  •  Entrez Gene ID: 6895
  •  Gene Symbol: TARBP2
  •  SwissProt: Q15633
  •  Chromosome Location: 12q13.13
  •  Unigene: 326