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VEGF (Vascular Endothelial Growth Factor)

Recombinant Rabbit Monoclonal Antibody [Clone VEGFA/7635R]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

Formalin-fixed, paraffin-embedded human liver stained with VEGF Recombinant Rabbit Monoclonal Antibody (VEGFA/7635R). HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human placenta stained with VEGF Recombinant Rabbit Monoclonal Antibody (VEGFA/7635R). Inset: PBS instead of primary antibody; secondary only negative control.

Formalin-fixed, paraffin-embedded human heart stained with VEGF Recombinant Rabbit Monoclonal Antibody (VEGFA/7635R). HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human liver stained with VEGF Recombinant Rabbit Monoclonal Antibody (VEGFA/7635R). HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human placenta stained with VEGF Recombinant Rabbit Monoclonal Antibody (VEGFA/7635R). Inset: PBS instead of primary antibody; secondary only negative control.

Formalin-fixed, paraffin-embedded human heart stained with VEGF Recombinant Rabbit Monoclonal Antibody (VEGFA/7635R). HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

Product Details

Synonyms

MVCD1; Vascular Endothelial Growth Factor A (VEGF-A); Vascular Permeability Factor (VPF); VEGFA

Positive Control

Tumor cells in astrocytomas, breast or ovarian carcinomas.

Known Applications & Suggested Dilutions

IHC-P More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment (around aa1-200) of human VEGFA protein (exact sequence is proprietary)

Cellular Localization

Cytoplasm. Cell surface. Extracellular (secreted).

Species Reactivity

Human.

Host / Ig Isotype

Rabbit / IgG1, kappa

Mol. Weight of Antigen

19-22kDa (reducing) and 38kDa-44kDa (non-reducing)

Specificity & Comments

This MAb recognizes proteins of 19-22kDa (reducing) and 38kDa-44kDa (non-reducing), identified as various isoforms of Vascular Endothelial Growth Factor or Vascular Permeability Factor (VEGF/VPF). It is highly specific to VEGF, which is a homodimeric, disulfide-linked glycoprotein with a close homology to platelet derived growth factor (PDGF). There are multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues. The smaller two isoforms, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression and metastasis.