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von Willebrand Factor / Factor VIII Related-Ag (Endothelial Marker)

Recombinant Mouse Monoclonal Antibody [Clone rVWF/1465]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

Formalin-fixed, paraffin-embedded human Tonsil stained with vWF Recombinant Mouse Monoclonal Antibody (rVWF/1465).

Western Blot Analysis A) Recombinant Protein (B) human lung lysate Using Recombinant Mouse Monoclonal Antibody (rVWF/1465).

SDS-PAGE Analysis Purified vWF Recombinant Mouse Monoclonal Antibody (rVWF/1465). Confirmation of Purity and Integrity of Antibody.

Formalin-fixed, paraffin-embedded human Tonsil stained with vWF Recombinant Mouse Monoclonal Antibody (rVWF/1465).

Western Blot Analysis A) Recombinant Protein (B) human lung lysate Using Recombinant Mouse Monoclonal Antibody (rVWF/1465).

SDS-PAGE Analysis Purified vWF Recombinant Mouse Monoclonal Antibody (rVWF/1465). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

Coagulation Factor VIII, Factor VIII Related Antigen, F8VWF, von Willebrand Antigen 2, von Willebrand Disease (vWD)

Positive Control

HUVEC cells or Tonsil

Known Applications & Suggested Dilutions

WB, IP, Immunohistology More Details

Western Blotting (0.5-1.0ug/ml)
Immunoprecipitation (0.5-1 �g/500ug protein lysate)
Immunohistology (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment of human vWF protein (aa1815-1939) (exact sequence is proprietary)

Cellular Localization

Cytoplasmic

Species Reactivity

Human. Others not known.

Host / Ig Isotype

Mouse / IgG1

Mol. Weight of Antigen

250kDa

Specificity & Comments

von Willebrand Factor (vWF) is a multimeric glycoprotein that is found in endothelial cells, plasma and platelets. It acts as a carrier protein for Factor VIII and promotes platelet adhesion and aggregation. vWF undergoes a variety of posttranslational modifications that influence the affinity and availability for Factor VIII, including cleavage of the propeptide and formation of N-terminal disulfide bonds. This antibody helps to establish the endothelial nature of some lesions of disputed histogenesis, e.g. Kaposi s sarcoma and cardiac myxoma. It is widely used for differentiating vascular lesions from those of other tissue differentiation within a panel of other vascular markers although not all tumors of endothelial differentiation contain this antigen.