Home >> Antibodies >> XRCC5 (Ku86 / Ku80) (Thyroid-Lupus Autoantigen)

XRCC5 (Ku86 / Ku80) (Thyroid-Lupus Autoantigen)

Recombinant Rabbit Monoclonal Antibody [Clone XRCC5/8093R]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

Product Details

Synonyms

ATP-dependent DNA helicase II 80kDa subunit; CTC box-binding factor 85kDa subunit (CTC85); CTCBF; DNA repair protein XRCC5; KARP1; Ku autoantigen 80kDa; Ku80; Ku86; KUB2; Lupus Ku autoantigen protein p86; Nuclear factor IV (NFIV); Thyroid-lupus autoantigen; TLAA; X-ray repair cross-complementing protein 5 (XRCC5)

Positive Control

HeLa, MOLT-4, MCF-7 or HT29 cells. Human thyroid or lymph node.

Known Applications & Suggested Dilutions

IHC-P More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment (around aa300-500) of human XRCC5 (exact sequence is proprietary)

Cellular Localization

Nucleus. Nucleoplasm.

Species Reactivity

Human.

Host / Ig Isotype

Rabbit / IgG1, kappa

Mol. Weight of Antigen

80-86kDa

Specificity & Comments

The Ku protein is localized in the nucleus and is composed of subunits referred to as Ku-70 (or p70) and Ku-86 or (p86) which is also known by the synonym Ku-80 or (p80). Ku was first described as an autoantigen to which antibodies were produced in a patient with scleroderma-polymyositis overlap syndrome, and was later found in the sera of patients with other rheumatic diseases. Ku has several functions, including cell signaling, DNA replication and transcriptional activation. Ku is involved in Pol II-directed transcription by virtue of its DNA binding activity; serving as the regulatory component of the DNA-associated protein kinase that phosphorylates Pol II and transcription factor Sp. Ku proteins also activate transcription from the U1 small nuclear RNA and the human transferrin receptor gene promoters.