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Home >> Antibodies >> Y14 / RBM8A

Y14 / RBM8A

Mouse Monoclonal Antibody [Clone PCRP-RBM8A-1B4]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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Flow Cytometric Analysis of PFA-fixed HeLa cells. Y14 / RBM8A Mouse Monoclonal Antibody (PCRP-RBM8A-1B4) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).

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Immunofluorescence Analysis of PFA-fixed HeLa cells using Y14 / RBM8A Mouse Monoclonal Antibody (PCRP-RBM8A-1B4) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).

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Analysis of Protein Array containing more than 19,000 full-length human proteins using Y14 / RBM8A Mouse Monoclonal Antibody (PCRP-RBM8A-1B4).

Product Details

Synonyms

Binder of OVCA1-1; BOV1A; BOV1B; BOV1C; HSPC114; MDS014; ZRNP1; Ribonucleoprotein RBM8A; RNA-binding protein 8A; RNA-binding protein Y14

Positive Control

HeLa or HepG2 cells.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human RBM8A protein

Cellular Localization

Nucleus. Nucleus speckle. Cytoplasm. Nucleocytoplasmic shuttling protein.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1

Mol. Weight of Antigen

24kDa

Specificity & Comments

Y14 Antibody is a high quality monoclonal Y14 antibody (also designated Y14 antibody) suitable for the detection of the Y14 protein of mouse, rat, human and Xenopus laevis origin. Y14 Antibody (4C4) is available as both the non-conjugated anti-Y14 antibody form, as well as multiple conjugated forms of anti-Y14 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor conjugates. The exon junction complex (EJC) is a multiprotein complex that assembles approximately 20-24 nucleotides upstream of exon-exon junctions in pre-mRNAs. It is involved in mRNA export, cytoplasmic localization, and nonsense-mediated mRNA decay. Members of the EJC include Y14, Aly/REF, Magoh, RNPS1, SRm160, and DEK. Aly/REF, Magoh, and Y14, identified as RBM8 in mouse and rat, make up the core of the EJC, and these proteins remain stably bound to spliced mRNAs in the cytoplasm until they are translated. Therefore, Y14, Aly/REF, and Magoh have the ability to communicate to the cytoplasm the processing history of the mRNA, including the position of the removed introns. The gene encoding human Y14 encodes three transcripts. Y14 is a ubiquitously expressed protein. Although Y14 shuttles to the cytoplasm, it is predominantly detected in the nucleus and is co-localized with oskar mRNA at the posterior pole of the cell.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Fehler, O., et al. 2014. An evolutionarily conserved interaction of tumor suppressor protein Pdcd4 with the poly(A)-binding protein contributes to translation suppression by Pdcd4. Nucleic Acids Res. 42: 11107-11118.

Bioinformatics

  •  Entrez Gene ID: 9939
  •  Gene Symbol: RBM8A
  •  SwissProt: Q9Y5S9
  •  Chromosome Location: 1q21.1
  •  Unigene: 728824