2 Union Square, Union City, CA 94587
contact@NeoBiotechnologies.com
510-376-5603

Home >> Antibodies >> YBX3 (Transcription Factor)

YBX3 (Transcription Factor)

Mouse Monoclonal Antibody [Clone PCRP-YBX3-2D12]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
img

Immunofluorescence Analysis of PFA-fixed HeLa cells stained using YBX3 Mouse Monoclonal Antibody (PCRP-YBX3-2D12) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).

img

Flow cytometric analysis of PFA-fixed HeLa cells. YBX3 Mouse Monoclonal Antibody (PCRP-YBX3-2D12) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).

img

Formalin-fixed, paraffin-embedded human bladder carcinoma stained with YBX3 Mouse Monoclonal Antibody (PCRP-YBX3-2D12). HIER: Tris/EDTA, pH9.0, 45min. 2°: HRP-polymer, 30min. DAB, 5min.

img

Analysis of Protein Array containing more than 19,000 full-length human proteins using YBX3 Mouse Monoclonal Antibody (PCRP-YBX3-2D12).

Product Details

Synonyms

Y-Box Binding Protein; ZONAB; CSDA1; CSDA; Single-Strand DNA-Binding Protein NF-GMB; Cold Shock Domain-Containing Protein A; Cold-Shock Domain Containing A1; Y-Box-Binding Protein; DNA-Binding Protein; DbpA; DBPA; ZO-1-Associated Nucleic Acid-Binding Protein; Cold Shock Domain Protein A; Cold-Shock Domain Protein A

Positive Control

HeLa or U87 cells. Human skeletal muscle, testis or bladder carcinoma.

Known Applications & Suggested Dilutions

IP, Flow Cyt, IF, Western Blot, IHC-P         More Details

Immunoprecipitation (1-2ug per 100-500ug of total protein (1ml of cell lysate))
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human YBX3 protein

Cellular Localization

Nucleus. Cytoplasm.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2b

Mol. Weight of Antigen

40.09kDa

Specificity & Comments

Predicted to enable DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Predicted to be involved in regulation of transcription by RNA polymerase II. Predicted to be active in nucleus. [provided by Alliance of Genome Resources, Apr 2022]

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Blackshaw S., et al. Nature methods 15.5 (2018 May): 330-338.

Bioinformatics

  •  Entrez Gene ID: 8531
  •  Gene Symbol: YBX3
  •  SwissProt: P16989
  •  Chromosome Location: 12p13.2
  •  Unigene: 221889