Home >> Antibodies >> YY1 (Transcription Factor)

YY1 (Transcription Factor)

Mouse Monoclonal Antibody [Clone YY1/5436]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

Formalin-fixed, paraffin-embedded human tonsil stained with YY1 Mouse Monoclonal (YY1/5436). Inset: PBS instead of primary antibody; secondary only negative control.

SDS-PAGE Analysis Purified YY1 Mouse Monoclonal Antibody (YY1/5436). Confirmation of Purity and Integrity of Antibody.

Western blot analysis of Daudi cell lysate using YY1 Mouse Monoclonal (YY1/5436).

Formalin-fixed, paraffin-embedded human tonsil stained with YY1 Mouse Monoclonal (YY1/5436). Inset: PBS instead of primary antibody; secondary only negative control.

SDS-PAGE Analysis Purified YY1 Mouse Monoclonal Antibody (YY1/5436). Confirmation of Purity and Integrity of Antibody.

Western blot analysis of Daudi cell lysate using YY1 Mouse Monoclonal (YY1/5436).

Product Details

Synonyms

CF1; Delta transcription factor; INO80 complex subunit S; NF E1; NFE1; Transcriptional repressor protein YY1; UCR motif DNA binding protein; UCRBP; Yin and yang 1; Yin and Yang 1 protein; YY-1; YY1 transcription factor

Positive Control

Human kidney, tonsil or cervix carcinoma tissues

Known Applications & Suggested Dilutions

Western Blot, IHC-P More Details

Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

His-tagged YY1 protein

Cellular Localization

Nucleus matrix. Associated with the nuclear matrix.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2, kappa

Mol. Weight of Antigen

40/68kDa

Specificity & Comments

The YY1 transcription factor, also known as NF-E1 (human) and Delta or UCRBP (mouse) is of interest due to its diverse effects on a wide variety of target genes. YY1 is broadly expressed in a wide range of cell types and contains four C-terminal zinc finger motifs of the Cys-Cys-His-His type and an unusual set of structural motifs at its N-terminal end. It binds to downstream elements in several vertebrate ribosomal protein genes, where it apparently acts positively to stimulate transcription and can act either negatively or positively in the context of the immunoglobulin k 3' enhancer and immunoglobulin heavy-chain �E1 site as well as the P5 promoter of the adenoassociated virus. It thus appears that YY1 is a bifunctional protein, capable of functioning as an activator in some transcriptional control elements and a repressor in others.